Coding

Part:BBa_K1705004

Designed by: Nathan Hunt   Group: iGEM15_MIT   (2015-09-17)

MoClo relE

relE is a gene for producing a toxin which prevents translation by cleaving mRNA; this makes it broadly applicable. Its counterpart antitoxin is relB. These two can be used to make a toxin-antitoxin system. We coupled this with quorum sensing to control population ratios between two strains in a bacterial co-culture.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 302


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Categories
Parameters
None